Anti-SMPD3 Antibody Picoband®

SKU: A04641

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SMPD3

Specificity:

Background: Sphingomyelin phosphodiesterase 3 is an enzyme that in humans is encoded by the SMPD3 gene. SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.

Immunogen: E.coli-derived human SMPD3 recombinant protein (Position: F184-D553). Human SMPD3 shares 88.9% amino acid (aa) sequence identity with both mouse and rat SMPD3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aubin, I., Adams, C. P., Opsahl, S., Septier, D., Bishop, C. E., Auge, N., Salvayre, R., Negre-Salvayre, A., Goldberg, M., Guenet, J.-L., Poirier, C. A deletion in the gene encoding sphingomyelin phosphodiesterase 3 (Smpd3) results in osteogenesis and dentinogenesis imperfecta in the mouse. Nature Genet. 37: 803-805, 2005. 2. Guenet, J. L., Stanescu, R., Maroteaux, P., Stanescu, V. Fragilitas ossium: a new autosomal recessive mutation in the mouse. J. Hered. 72: 440-441, 1981. 3. Hofmann, K., Tomiuk, S., Wolff, G., Stoffel, W. Cloning and characterization of the mammalian brain-specific, Mg(2+)-dependent neutral sphingomyelinase. Proc. Nat. Acad. Sci. 97: 5895-5900, 2000.

Uniprot ID: Q9NY59

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 55512

Protein Name: Sphingomyelin phosphodiesterase 3

Gene Full Name: sphingomyelin phosphodiesterase 3

Synonyms: SMPD3; Sphingomyelin phosphodiesterase 3; Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II