Anti-SIVA/SIVA1 Antibody Picoband®

SKU: A05419-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SIVA1

Specificity:

Background: Apoptosis regulatory protein Siva is a protein that in humans is encoded by the SIVA1 gene. This gene encodes an E3 ubiquitin ligase that regulates cell cycle progression, cell proliferation and apoptosis. The N-terminus of this protein binds to the cytoplasmic tail of the CD27 antigen, a member of the tumor necrosis factor receptor (TNFR) superfamily. In response to UV radiation-induced DNA damage, this protein has been shown to mediate the ubiquitination of proliferating cell nuclear antigen (PCNA), an important step in translesion DNA synthesis.

Immunogen: E.coli-derived human SIVA/SIVA1 recombinant protein (Position: M1-T141).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Nestler, M., Martin, U., Hortschansky, P., Saluz, H.-P., Henke, A., Munder, T. The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22. Molec. Cell. Biochem. 287: 147-155, 2006. 2. Prasad, K. V. S., Ao, Z., Yoon, Y., Wu, M. X., Rizk, M., Jacquot, S., Schlossman, S. F. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Nat. Acad. Sci. 94: 6346-6351, 1997. 3. Py, B., Slomianny, C., Auberger, P., Petit, P. X., Benichou, S. Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway. J. Immun. 172: 4008-4017, 2004.

Uniprot ID: O15304

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 19 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 10572

Protein Name: Apoptosis regulatory protein Siva

Gene Full Name: SIVA1 apoptosis inducing factor

Synonyms: SIVA1; SIVA; Apoptosis regulatory protein Siva; CD27-binding protein; CD27BP