Anti-SHROOM2 Antibody Picoband®

SKU: A10015-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SHROOM2

Specificity:

Background: Shroom family member 2 is a protein that in humans is encoded by the SHROOM2 gene. This gene represents the human homolog of Xenopus laevis apical protein (APX) gene, which is implicated in amiloride-sensitive sodium channel activity. It is expressed in endothelial cells and facilitates the formation of a contractile network within endothelial cells. Depletion of this gene results in an increase in endothelial sprouting, migration, and angiogenesis. This gene is highly expressed in the retina, and is a strong candidate for ocular albinism type 1 syndrome. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human SHROOM2 recombinant protein (Position: R427-H1138).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dietz, M. L., Bernaciak, T. M., Vendetti, F., Kielec, J. M., Hildebrand, J. D. Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins. J. Biol. Chem. 281: 20542-20554, 2006. 2. Dinulos, M. B., Bassi, M. T., Rugarli, E. I., Chapman, V., Ballabio, A., Disteche, C. M. A new region of conservation is defined between human and mouse X chromosomes. Genomics 35: 244-247, 1996. 3. Schiaffino, M. V., Bassi, M. T., Rugarli, E. I., Renieri, A., Galli, L., Ballabio, A. Cloning of a human homologue of the Xenopus laevis APX gene from the ocular albinism type 1 critical region. Hum. Molec. Genet. 4: 373-382, 1995.

Uniprot ID: Q13796

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 180 kDa

Calculated Molecular Weight: 16799 MW

Gene ID: 357

Protein Name: Protein Shroom2

Gene Full Name: shroom family member 2

Synonyms: SHROOM2; APXL; Protein Shroom2; Apical-like protein; Protein APXL