Anti-SF3A3 Antibody Picoband®

SKU: A10671-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SF3A3

Specificity:

Background: Splicing factor 3A subunit 3 is a protein that in humans is encoded by the SF3A3 gene. This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants

Immunogen: E.coli-derived human SF3A3 recombinant protein (Position: E123-Q472).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chiara, M. D., Champion-Arnaud, P., Buvoli, M., Nadal-Ginard, B., Reed, R. Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114. Proc. Nat. Acad. Sci. 91: 6403-6407, 1994. 2. Gross, M. B. Personal Communication. Baltimore, Md. 4/4/2014. 3. Kramer, A., Legrain, P., Mulhauser, F., Groning, K., Brosi, R., Bilbe, G. Splicing factor SF3a60 is the mammalian homologue of PRP9 of S. cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. Nucleic Acids Res. 22: 5223-5228, 1994.

Uniprot ID: Q12874

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 59 kDa

Calculated Molecular Weight: 29370 MW

Gene ID: 10946

Protein Name: Splicing factor 3A subunit 3

Gene Full Name: splicing factor 3a subunit 3

Synonyms: PRP9; PRPF9; SAP 61; SAP61; SF3A3; SF3a60; Splicing factor 3A subunit 3