Anti-SDCCAG1/NEMF Antibody Picoband®

SKU: A09039-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NEMF

Specificity:

Background: Nuclear export mediator factor (NEMF) is a protein that in humans is encoded by the NEMF gene. This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor.

Immunogen: E.coli-derived human SDCCAG1/NEMF recombinant protein (Position: H81-R607).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ahmed, A., Wang, M., Bergant, G., Maroofian, R., Zhao, R., Alfadhel, M., Nashabat, M., AlRifai, M. T., Eyaid, W., Alswaid, A., Beetz, C., Qin, Y., and 21 others. Biallelic loss-of-function variants in NEMF cause central nervous system impairment and axonal polyneuropathy. Hum. Genet. 140: 579-592, 2021. 2. Anazi, S., Maddirevula, S., Faqeih, E., Alsedairy, H., Alzahrani, F., Shamseldin, H. E., Patel, N., Hashem, M., Ibrahim, N., Abdulwahab, F., Ewida, N., Alsaif, H. S., and 36 others. Clinical genomics expands the morbid genome of intellectual disability and offers a high diagnostic yield. Molec. Psychiat. 22: 615-624, 2017. 3. Carbonnelle, D., Liehr, T., Jacquot, C., Masson, D., Lustenberger, P., Denis, M. G., Roussakis, C. Assignment of the serologically defined colon cancer antigen 1 gene (SDCCAG1) to human chromosome band 14q22 by in situ hybridization. Cytogenet. Cell Genet. 86: 248-249, 1999.

Uniprot ID: O60524

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight:

Gene ID: 9147

Protein Name: Ribosome quality control complex subunit NEMF

Gene Full Name: nuclear export mediator factor

Synonyms: NEMF; NY CO 1; SDCCAG1