Anti-SCRIBBLE Antibody Picoband®

SKU: A01651

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
ELISA, 0.1-0.5μg/ml

Gene Name: SCRIB

Specificity: No cross reactivity with other proteins.

Background: SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.

Immunogen: E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. L E Dow; J S Kauffman; J Caddy; A S Peterson; S M Jane; S M Russell & P O Humbert (April 2007). The tumour-suppressor Scribble dictates cell polarity during ed epithelial migration: regulation of Rho GTPase recruitment to the leading edge. Oncogene. 26 (16): 2272–82. 2. Yi Qin; Christopher Capaldo; Barry M. Gumbiner & Ian G. Macara (December 2005). The mammalian SCRIB polarity protein regulates epithelial cell adhesion and migration through E-cadherin. JCB. 171 (6): 1061–71.

Uniprot ID: Q14160

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 240 kDa

Calculated Molecular Weight: 50514 MW

Gene ID: 23513

Protein Name: Protein scribble homolog

Gene Full Name: scribble planar cell polarity protein

Synonyms: CRIB1; hScrib; KIAA0147; LAP4; Protein LAP4; Protein scribble homolog; SCRB1; SCRIB; SCRIB1; Scribble; Vartul