Anti-SCAP Antibody Picoband®

SKU: A04507-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml

Gene Name: SCAP

Specificity:

Background: This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human SCAP recombinant protein (Position: F1224-D1279). Human SCAP shares 100% amino acid (aa) sequence identity with both mouse and rat SCAP.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Brown, A. J., Sun, L., Feramisco, J. D., Brown, M. S., Goldstein, J. L. Cholesterol addition to ER membranes alters conformation of SCAP, the SREBP escort protein that regulates cholesterol metabolism. Molec. Cell 10: 237-245, 2002. 2. Cao, H., Miskie, B. A., Hegele, R. A. Functional promoter polymorphism in SREBP cleavage-activating protein (SCAP). J. Hum. Genet. 47: 492-496, 2002. 3. DeBose-Boyd, R. A., Brown, M. S., Li, W.-P., Nohturfft, A., Goldstein, J. L., Espenshade, P. J. Transport-dependent proteolysis of SREBP: relocation of Site-1 protease from Golgi to ER obviates the need for SREBP transport to Golgi. Cell 99: 703-712, 1999.

Uniprot ID: Q12770

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 140 kDa

Gene ID: 22937

Protein Name: Sterol regulatory element-binding protein cleavage-activating protein

Gene Full Name: SREBF chaperone

Synonyms: SCAP; KIAA0199; PSEC0227; Sterol regulatory element-binding protein cleavage-activating protein; SCAP; SREBP cleavage-activating protein