Anti-RNF111 Antibody Picoband®

SKU: A05708-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RNF111

Specificity:

Background: E3 ubiquitin-protein ligase Arkadia is an enzyme that in humans is encoded by the RNF111 gene. The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived human RNF111 recombinant protein (Position: F134-S994).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Episkopou, V., Arkell, R., Timmons, P. M., Walsh, J. J., Andrew, R. L., Swan, D. Induction of the mammalian node requires Arkadia function in the extraembryonic lineages. Nature 410: 825-830, 2001. 2. Niederlander, C., Walsh, J. J., Episkopou, V., Jones, C. M. Arkadia enhances nodal-related signalling to induce mesendoderm. Nature 410: 830-834, 2001.

Uniprot ID: Q6ZNA4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 109 kDa

Calculated Molecular Weight: 109 kDa

Gene ID: 54778

Protein Name: E3 ubiquitin-protein ligase Arkadia

Gene Full Name: ring finger protein 111

Synonyms: RNF111; E3 ubiquitin-protein ligase Arkadia; RING finger protein 111; hRNF111; RING-type E3 ubiquitin transferase Arkadia