Anti-RING2/RING1B/RNF2 Antibody Picoband®

SKU: A01209-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RNF2

Specificity:

Background: E3 ubiquitin-protein ligase RING2 is an enzyme that in humans is encoded by the RNF2 gene. Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Immunogen: E.coli-derived human RING2/RING1B/RNF2 recombinant protein (Position: M1-K336).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Entrez Gene: RNF2 ring finger protein 2. 2. Lee SJ, Choi JY, Sung YM, Park H, Rhim H, Kang S (August 2001). E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme. FEBS Lett 503 (1): 61–4. 3. Tuckfield, Annabel; Clouston David R; Wilanowski Tomasz M; Zhao Lin-Lin; Cunningham John M; Jane Stephen M (March 2002). Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors. Mol. Cell. Biol. (United States) 22 (6): 1936–46.

Uniprot ID: Q99496

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 123799 MW

Gene ID: 6045

Protein Name: E3 ubiquitin-protein ligase RING2

Gene Full Name: ring finger protein 2

Synonyms: BAP 1; BAP1; DING; HIP2 interacting protein 3; HIPI3; Protein DinG; RING finger protein 1B; ring finger protein 2; RING finger protein BAP 1; RING1B; RING2; RNF2