Anti-Rel B/RELB Antibody Picoband®

SKU: PA1803

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: RELB

Specificity: No cross reactivity with other proteins.

Background: RELB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. The International Radiation Hybrid Mapping Consortium assigned the RELB gene to chromosome 19. By RT-PCR and immunocytochemical analyses, Clark et al. (1999) showed that RELB expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al. (2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both Relb -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al. (2008) concluded that the alternative NF-kappa-B pathway, via RELB, plays an essential and unique role in RANKL signaling toward osteoclast development.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rel B, identical to the related mouse sequence.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Clark, G. J., Gunningham, S., Troy, A., Vuckovic, S., Hart, D. N.Expression of the RelB transcription factor correlates with the activation of human dendritic cells.Immunology 98: 189-196, 1999. 2. Ruben, S. M., Klement, J. F., Coleman, T. A., Maher, M., Chen, C.-H., Rosen, C. A.I-Rel: a novel rel-related protein that inhibits NF-kappa-B transcriptional activity.Genes Dev. 6: 745-760, 1992. 3. Vaira, S., Johnson, T., Hirbe, A. C., Alhawagri, M., Anwisye, I., Sammut, B., O'Neal, J., Zou, W., Weilbaecher, K. N., Faccio, R., Novack, D. V. RelB is the NF-kappa-B subunit downstream of NIK responsible for osteoclast differentiation.Proc. Nat. Acad. Sci. 105: 3897-3902, 2008.

Uniprot ID: Q01201

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 23 kDa

Calculated Molecular Weight: 62134 MW

Gene ID: 5971

Protein Name: Transcription factor RelB

Gene Full Name: RELB proto-oncogene, NF-kB subunit

Synonyms: I REL; IREL; RELB; Transcription factor RelB