Anti-Raf1 Antibody Picoband®

SKU: A00446-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: RAF1

Specificity: No cross reactivity with other proteins.

Background: RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinasekinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds ly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Immunogen: E. coli-derived human Raf1 recombinant protein (Position: Y364-Q451). Human Raf1 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Raf1.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Li P, Wood K, Mamon H, Haser W, Roberts T (February 1991). Raf-1: a kinase currently without a cause but not lacking in effects. Cell. 64 (3): 479–82. 2. Rapp UR, Goldsborough MD, Mark GE, Bonner TI, Groffen J, Reynolds FH, Stephenson JR (July 1983). Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc. Natl. Acad. Sci. U.S.A. 80 (14): 4218–22.

Uniprot ID: P04049

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 73 kDa

Calculated Molecular Weight: 73052 MW

Gene ID: 5894

Protein Name: RAF proto-oncogene serine/threonine-protein kinase

Gene Full Name: Raf-1 proto-oncogene, serine/threonine kinase

Synonyms: c Raf; CRAF; NS5; Proto oncogene c RAF; RAF; Raf 1; RAF1