Anti-RAD51AP1 Antibody Picoband®

SKU: A06705-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IF,IHC,ICC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RAD51AP1

Specificity:

Background: The RAD51AP1 gene encodes a protein known as RAD51-associated protein 1, which plays a crucial role in homologous recombination, a DNA repair pathway essential for maintaining genomic integrity. RAD51AP1 interacts with RAD51, a key protein involved in homologous recombination, and promotes the assembly of RAD51 nucleoprotein filaments on single-stranded DNA, facilitating the search for homologous sequences and strand exchange. This process is vital for repairing DNA double-strand breaks and stalled replication forks, as well as for meiotic recombination and telomere maintenance. Dysregulation of RAD51AP1 expression or function has been implicated in cancer development and progression, as defects in homologous recombination can lead to genomic instability and oncogenesis. Understanding the molecular mechanisms underlying RAD51AP1-mediated homologous recombination is crucial for deciphering its role in DNA repair and genome maintenance, as well as for developing targeted therapies for cancer and other diseases associated with DNA damage.

Immunogen: E.coli-derived human RAD51AP1 recombinant protein (Position: M1-K303).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kovalenko, O. V., Golub, E. I., Bray-Ward, P., Ward, D. C., Radding, C. M. A novel nucleic acid-binding protein that interacts with human Rad51 recombinase. Nucleic Acids Res. 25: 4946-4953, 1997. 2. Mizuta, R., LaSalle, J. M., Cheng, H.-L., Shinohara, A., Ogawa, H., Copeland, N., Jenkins, N. A., Lalande, M., Alt, F. W. RAB22 and RAB163/mouse BRCA2: proteins that specifically interact with the RAD51 protein. Proc. Nat. Acad. Sci. 94: 6927-6932, 1997. 3. Modesti, M., Budzowska, M., Baldeyron, C., Demmers, J. A. A., Ghirlando, R., Kanaar, R. RAD51AP1 is a structure-specific DNA binding protein that stimulates joint molecule formation during RAD51-mediated homologous recombination. Molec. Cell 28: 468-481, 2007.

Uniprot ID: Q96B01

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 38 kDa

Calculated Molecular Weight: 38 kDa

Gene ID: 10635

Protein Name: RAD51-associated protein 1

Gene Full Name: RAD51 associated protein 1

Synonyms: RAD51AP1; PIR51; RAD51-associated protein 1; RAD51-interacting protein