Anti-PSTPIP1 Antibody Picoband®

SKU: A04844-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PSTPIP1

Specificity:

Background: Proline-serine-threonine phosphatase-interacting protein 1 is an enzyme that in humans is encoded by the PSTPIP1 gene. This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and s them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response.

Immunogen: E.coli-derived human PSTPIP1 recombinant protein (Position: M1-D394). Human PSTPIP1 shares 87.3% amino acid (aa) sequence identity with mouse PSTPIP1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cong, F., Spencer, S., Cote, J.-F., Wu, Y., Tremblay, M. L., Lasky, L. A., Goff, S. P. Cytoskeletal protein PSTPIP1 s the PEST-type protein tyrosine phosphatase to the c-Abl kinase to mediate Abl dephosphorylation. Molec. Cell 6: 1413-1423, 2000. 2. Gross, M. B. Personal Communication. Baltimore, Md. 2/17/2015. 3. Li, J., Nishizawa, K., An, W., Hussey, R. E., Lialios, F. E., Salgia, R., Sunder-Plassmann, R., Reinherz, E. L. A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion. EMBO J. 17: 7320-7336, 1998.

Uniprot ID: O43586

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 45 kDa

Calculated Molecular Weight:

Gene ID: 9051

Protein Name: Proline-serine-threonine phosphatase-interacting protein 1

Gene Full Name: proline-serine-threonine phosphatase interacting protein 1

Synonyms: CD2 binding protein 1; CD2BP1; CD2BP1L; CD2BP1S; H PIP; PAPAS; PSTPIP; PSTPIP1