Anti-PSMF1 Antibody Picoband®

SKU: A08650-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PSMF1

Specificity:

Background: Proteasome inhibitor PI31 subunit is a protein that in humans is encoded by the PSMF1 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Immunogen: E.coli-derived human PSMF1 recombinant protein (Position: M1-L271).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. An, H., Yang, L., Wang, C., Gan, Z., Gu, H., Zhang, T., Huang, X., Liu, Y., Li, Y., Chang, S.-J., Lai, J., Li, Y.-B., Chen, S., Sun, F.-L. Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage. Molec. Cell. Biol. 37: e00419-16, 2017. Note: Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 1/30/2018. 3. Li, X., Thompson, D., Kumar, B., DeMartino, G. N. Molecular and cellular roles of PI31 (PSMF1) protein in regulation of proteasome function. J. Biol. Chem. 289: 17392-17405, 2014.

Uniprot ID: Q92530

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 30 kDa

Calculated Molecular Weight: 36881 MW

Gene ID: 9491

Protein Name: Proteasome inhibitor PI31 subunit

Gene Full Name: proteasome inhibitor subunit 1

Synonyms: hPI31; PI31; PSMF1