Anti-PRDM15 Antibody Picoband®

SKU: A13548-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRDM15

Specificity:

Background: PR/SET domain 15 is a protein that in humans is encoded by the PRDM15 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and promoter-specific chromatin binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II; regulation of signal transduction; and regulation of stem cell division. Located in nuclear body.

Immunogen: E.coli-derived human PRDM15 recombinant protein (Position: D24-Q1494).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Mzoughi, S., Zhang, J., Hequet, D., Teo, S. X., Fang, H., Xing, Q. R., Bezzi, M., Seah, M. K. Y., Ong, S. L. M., Shin, E. M., Wollmann, H., Wong, E. S. M., Al-Haddawi, M., Stewart, C. L., Tergaonkar, V., Loh, Y.-H., Dunn, N. R., Messerschmidt, D. M., Guccione, E. PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAP-ERK signaling. Nature Genet. 49: 1354-1363, 2017. 2. Shibuya, K., Kudoh, J., Okui, M., Shimizu, N. Identification of a novel zinc finger protein gene (ZNF298) in the GAP2 of human chromosome 21q. Biochem. Biophys. Res. Commun. 332: 557-568, 2005.

Uniprot ID: P57071

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 169 kDa

Calculated Molecular Weight: 64099 MW

Gene ID: 63977

Protein Name: PR domain zinc finger protein 15

Gene Full Name: PR/SET domain 15

Synonyms: C21orf83; PFM15; PR domain containing 15; PRDM15, Zinc finger protein 298; ZNF298