Anti-PPP3R1 Antibody Picoband®

SKU: A06469-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PPP3R1

Specificity:

Background: Calcineurin, the Ca(2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha(PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase(calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast.

Immunogen: E.coli-derived human PPP3R1 recombinant protein (Position: E48-V170).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Giri, P.; Higuchi, S.; Kincaid, R. L. : Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit. Biochem. Biophys. Res. Commun. 181: 252-258, 1991. 2. Sun, L.; Blair, H. C.; Peng, Y.; Zaidi, N.; Adebanjo, O. A.; Wu, X. B.; Wu, X. Y.; Iqbal, J.; Epstein, S.; Abe, E.; Moonga, B. S.; Zaidi, M. : Calcineurin regulates bone formation by the osteoblast. Proc. Nat. Acad. Sci. 102: 17130-17135, 2005. 3. Wang, M. G.; Yi, H.; Guerini, D.; Klee, C. B.; McBride, O. W. : Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21-q22 and 2p16-p15 respectively. Cytogenet. Cell Genet. 72: 236-241, 1996.

Uniprot ID: P63098

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 15 kDa

Calculated Molecular Weight:

Gene ID: 5534

Protein Name: Calcineurin subunit B type 1

Gene Full Name: protein phosphatase 3 regulatory subunit B, alpha

Synonyms: Calcineurin subunit B type 1; CALNB1; CNA2; CNB; CNB1; PPP3R1