Anti-PPAR alpha/PPARA Antibody Picoband®

SKU: A00600-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PPARA

Specificity:

Background: Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

Immunogen: E.coli-derived human PPAR alpha/PPARA recombinant protein (Position: M1-L426).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Sher T, Yi HF, McBride OW, Gonzalez FJ (June 1993). cDNA cloning, chromosomal mapping, and functional characterization of the human peroxisome proliferator activated receptor. Biochemistry 32 (21): 5598–604. doi:10.1021/bi00072a015. PMID 7684926. 2. Kersten S, Seydoux J, Peters JM, Gonzalez FJ, Desvergne B, Wahli W. (June 1999). Peroxisome proliferator-activated receptor alpha mediates the adaptive response to fasting.. J Clin Invest. 103 (11): 1489–98. doi:10.1172/JCI6223. PMC 408372. PMID 10359558. 3. Jung, D., Fried, M., Kullak-Ublick, G. A.Human apical sodium-dependent bile salt transporter gene (SLC10A2) is regulated by the peroxisome proliferator-activated receptor alpha.J. Biol. Chem.277: 30559-30566, 2002.

Uniprot ID: Q07869

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight:

Gene ID: 5465

Protein Name: Peroxisome proliferator-activated receptor alpha

Gene Full Name: peroxisome proliferator activated receptor alpha

Synonyms: hPPAR; NR1C1; PPAR; PPAR alpha; PPARA; PPARα