Anti-POLG2 Antibody Picoband®

SKU: A06012-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: POLG2

Specificity:

Background: This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.

Immunogen: E.coli-derived human POLG2 recombinant protein (Position: Q29-E434). Human POLG2 shares 78.2% amino acid (aa) sequence identity with mouse POLG2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Carrodeguas, J. A., Bogenhagen, D. F. Protein sequences conserved in prokaryotic aminoacyl-tRNA synthetases are important for the activity of the processivity factor of human mitochondrial DNA polymerase. Nucleic Acids Res. 28: 1237-1244, 2000. 2. Carrodeguas, J. A., Theis, K., Bogenhagen, D. F., Kisker, C. Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol-gamma-B, functions as a homodimer. Molec. Cell 7: 43-54, 2001. 3. Dosekova, P., Dubiel, A., Karlowicz, A., Zietkiewicz, S., Rydzanicz, M., Habalova, V., Pienkowski, V. M., Skirkova, M., Han, V., Mosejova, A., Gdovinova, Z., Kaliszewska, M., Tonska, K., Szymanski, M. R., Skorvanek, M., Ploski, R. Whole exome sequencing identifies a homozygous POLG2 missense variant in an adult patient presenting with optic atrophy, movement disorders, premature ovarian failure and mitochondrial DNA depletion. Europ. J. Med. Genet. 63: 103821, 2020.

Uniprot ID: Q9UHN1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 55 kDa

Gene ID: 11232

Protein Name: DNA polymerase subunit gamma-2

Gene Full Name: DNA polymerase gamma 2, accessory subunit

Synonyms: HP55; MTPOLB; p55; PEOA4; POLB; POLG BETA; POLG2; POLGB