Anti-POLD1 Antibody Picoband®

SKU: A03720-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,IP,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: POLD1

Specificity:

Background: This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

Immunogen: E.coli-derived human POLD1 recombinant protein (Position: D25-D1068). Human POLD1 shares 89.5% and 90.2% amino acid (aa) sequence identity with mouse and rat POLD1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bellido, F., Pineda, M., Aiza, G., Valdes-Mas, R., Navarro, M., Puente, D. A., Pons, T., Gonzalez, S., Iglesias, S., Darder, E., Pinol, V., Soto, J. L., Valencia, A., Blanco, I., Urioste, M., Brunet, J., Lazaro, C., Capella, G., Puente, X. S., Valle, L. POLE and POLD1 mutations in 529 kindred with familial colorectal cancer and/or polyposis: review of reported cases and recommendations for genetic testing and surveillance. Genet. Med. 18: 325-332, 2016. 2. Chang, L.-S., Zhao, L., Zhu, L., Chen, M.-L., Lee, M. Y. W. T. Structure of the gene for the catalytic subunit of human DNA polymerase delta (POLD1). Genomics 28: 411-419, 1995. 3. Chung, D. W., Zhang, J., Tan, C.-K., Davie, E. W., So, A. G., Downey, K. M. Primary structure of the catalytic subunit of human DNA polymerase delta and chromosomal location of the gene. Proc. Nat. Acad. Sci. 88: 11197-11201, 1991.

Uniprot ID: P28340

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 124 kDa

Calculated Molecular Weight: 124 kDa

Gene ID: 5424

Protein Name: DNA polymerase delta catalytic subunit

Gene Full Name: DNA polymerase delta 1, catalytic subunit

Synonyms: CDC2; POLD; POLD1