Anti-PLOD1 Antibody Picoband®

SKU: A05322-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLOD1

Specificity:

Background: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human PLOD1 recombinant protein (Position: K23-A640).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Al-Gazali, L. I., Bakalinova, D., Varady, E., Scorer, J., Nork, M. Further delineation of Nevo syndrome. J. Med. Genet. 34: 366-370, 1997. 2. Brinckmann, J., Acil, Y., Feshchenko, S., Katzer, E., Brenner, R., Kulozik, A., Kugler, S. Ehlers-Danlos syndrome type VI: lysyl hydroxylase deficiency due to a novel point mutation (W612C). Arch. Derm. Res. 290: 181-186, 1998. 3. Dembure, P. P., Janko, A. R., Priest, J. H., Elsas, L. J. Ascorbate regulation of collagen biosynthesis in Ehlers-Danlos syndrome, type VI. Metabolism 36: 687-691, 1987.

Uniprot ID: Q02809

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 84 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 5351

Protein Name: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene Full Name: procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Synonyms: PLOD1; LLH; PLOD; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1; LH1