Anti-PLEKHG4 Antibody Picoband®

SKU: A10252-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLEKHG4

Specificity:

Background: Puratrophin-1 is a protein that in humans is encoded by the PLEKHG4 gene. The protein encoded by this gene can function as a guanine nucleotide exchange factor (GEF) and may play a role in intracellular signaling and cytoskeleton dynamics at the Golgi apparatus. Polymorphisms in the region of this gene have been found to be associated with spinocerebellar ataxia in some study populations. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PLEKHG4 recombinant protein (Position: R22-E1140).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Amino, T., Ishikawa, K., Toru, S., Ishiguro, T., Sato, N., Tsunemi, T., Murata, M., Kobayashi, K., Inazawa, J., Toda, T., Mizusawa, H. Redefining the disease locus of 16q22.1-linked autosomal dominant cerebellar ataxia. J. Hum. Genet. 52: 643-649, 2007. 2. Ishikawa, K., Toru, S., Tsunemi, T., Li, M., Kobayashi, K., Yokota, T., Amino, T., Owada, K., Fujigasaki, H., Sakamoto, M., Tomimitsu, H., Takashima, M., and 15 others. An autosomal dominant cerebellar ataxia linked to chromosome 16q22.1 is associated with a single-nucleotide substitution in the 5-prime untranslated region of the gene encoding a protein with spectrin repeat and Rho guanine-nucleotide exchange-factor domains. Am. J. Hum. Genet. 77: 280-296, 2005. 3. Lim, J., Hao, T., Shaw, C., Patel, A. J., Szabo, G., Rual, J.-F., Fisk, C. J., Li, N., Smolyar, A., Hill, D. E., Barabasi, A.-L., Vidal, M., Zoghbi, H. Y. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 125: 801-814, 2006.

Uniprot ID: Q58EX7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 25894

Protein Name: Puratrophin-1

Gene Full Name: pleckstrin homology and RhoGEF domain containing G4

Synonyms: Puratrophin-1; Pleckstrin homology domain-containing family G member 4; PH domain-containing family G member 4; Purkinje cell atrophy-associated protein 1; PLEKHG4; PRTPHN1