Anti-PKC iota/PRKCI Antibody Picoband®

SKU: PB9321

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: PRKCI

Specificity: No cross reactivity with other proteins.

Background: Protein kinase C iota type, also called PKCI, is an enzyme that in humans is encoded by the PRKCI gene. This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. PRKCI is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKCI is also a hedgehog target gene that forms a positive feedback loop with GLI and exists at increased levels in basal cell carcinomas.

Immunogen: E.coli-derived human PKC iota recombinant protein (Position: D21-Q214). Human PKC iota shares 96% and 97% amino acid (aa) sequence identity with mouse and rat PKC iota, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Atwood, S. X., Li, M., Lee, A., Tang, J. Y., Oro, A. E. GLI activation by atypical protein kinase C lambda/iota regulates the growth of basal cell carcinomas. Nature 494: 484-488, 2013. 2. De Donato, M., Gallagher Jr., D. S., Davis, S. K, Stelly, D. M., Taylor, J.F. The assignment ofPRKCI to bovine chromosome 1q34-q36 by FISH suggests a new assignment to human chromosome 3. Cytogenet. Cell Genet. 95: 79-81, 2001. 3. Mazzarella R, Ciccodicola A, Esposito T, Arcucci A, Migliaccio C, Jones C, Schlessinger D, D'Urso M, D'Esposito M (August 1995). Human protein kinase C Iota gene (PRKCI) is closely linked to the BTK gene in Xq21.3. Genomics 26(3): 629–31.

Uniprot ID: P41743

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 68262 MW

Gene ID: 5584

Protein Name: Protein kinase C iota type

Gene Full Name: protein kinase C iota

Synonyms: aPKC lambda/iota; DXS1179E; PKC iota; PKCI; PRKC lambda/iota; PRKCI; Protein kinase C iota type; protein kinase C; iota