Anti-PELI2 Antibody Picoband®

SKU: A10176-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PELI2

Specificity:

Background: Protein pellino homolog 2 is a protein that in humans is encoded by the PELI2 gene. PELI2 is a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. It is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. It mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. It may be important for LPS- and IL1B-induced MAP3K7- dependent, but not MAP3K3-dependent, NF-kappa-B activation. And it can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1.

Immunogen: E.coli-derived human PELI2 recombinant protein (Position: D118-D420). Human PELI2 shares 97.4% amino acid (aa) sequence identity with mouse?PELI2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Jensen, L. E., Whitehead, A. S. Pellino2 activates the mitogen activated protein kinase pathway. FEBS Lett. 545: 199-202, 2003. 2. Kim, T. W., Yu, M., Zhou, H., Cui, W., Wang, J., DiCorleto, P., Fox, P., Xiao, H., Li, X. Pellino 2 is critical for Toll-like receptor/interleukin-1 receptor (TLR/IL-1R)-mediated post-transcriptional control. J. Biol. Chem. 287: 25686-25695, 2012. 3. Liu, Y., Dong, W., Chen, L., Xiang, R., Xiao, H., De. G., Wang, Z., Qi, Y. BCL10 mediates lipopolysaccharide/Toll-like receptor-4 signaling through interaction with Pellino2. J. Biol. Chem. 279: 37436-37444, 2004.

Uniprot ID: Q9HAT8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 57161

Protein Name: E3 ubiquitin-protein ligase pellino homolog 2

Gene Full Name: pellino E3 ubiquitin protein ligase family member 2

Synonyms: PELI2; E3 ubiquitin-protein ligase pellino homolog 2; Pellino-2; RING-type E3 ubiquitin transferase pellino homolog 2