Anti-PDE8A Antibody Picoband®

SKU: A05304-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PDE8A

Specificity:

Background: High affinity cAMP-specific and IBMX-insensitive 3', 5'-cyclic phosphodiesterase 8A is an enzyme that in humans is encoded by the PDE8A gene. The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human PDE8A recombinant protein (Position: R32-E495). Human PDE8A shares 80.5% amino acid (aa) sequence identity with mouse PDE8A.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fisher, D. A., Smith, J. F., Pillar, J. S., St. Denis, S. H., Cheng, J. B. Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase. Biochem. Biophys. Res. Commun. 246: 570-577, 1998. 2. Hetman, J. M., Soderling, S. H., Glavas, N. A., Beavo, J. A. Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase. Proc. Nat. Acad. Sci. 97: 472-476, 2000. 3. Vasta, V., Shimizu-Albergine, M., Beavo, J. A. Modulation of Leydig cell function by cyclic nucleotide phosphodiesterase 8A. Proc. Nat. Acad. Sci. 103: 19925-19930, 2006.

Uniprot ID: O60658

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 93 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 5151

Protein Name: High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Gene Full Name: phosphodiesterase 8A

Synonyms: PDE8A; High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A