Anti-PCNA Antibody Picoband® (monoclonal, 2G2)

SKU: M00125-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human

Gene Name: PCNA

Specificity:

Background: Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Immunogen: E.coli-derived human PCNA recombinant protein (Position: M1-S261).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Moldovan GL, Pfander B, Jentsch S (May 18, 2007). PCNA, the maestro of the replication fork. Cell. 129 (4): 665–79. 2. Warbrick E (Mar 1998). PCNA binding through a conserved motif. BioEssays. 20 (3): 195–9. 3. Mailand N, Gibbs-Seymour I, Bekker-Jensen S (May 2013). Regulation of PCNA-protein interactions for genome stability. Nature Reviews. Molecular Cell Biology. 14 (5): 269–82.

Uniprot ID: P12004

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 2G2

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight:

Gene ID: 5111

Protein Name: Proliferating cell nuclear antigen

Gene Full Name: proliferating cell nuclear antigen

Synonyms: PCNA