Anti-PARP10 Antibody Picoband®

SKU: A08514-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PARP10

Specificity:

Background: Poly [ADP-ribose] polymerase 10 is an enzyme that in humans is encoded by the PARP10 gene. Poly(ADP-ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP-ribose to histones. PARPs can also function as transcriptional cofactors.

Immunogen: E.coli-derived human PARP10 recombinant protein (Position: E14-E251). Human PARP10 shares 76.5% amino acid (aa) sequence identity with mouse PARP10.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kleine, H., Poreba, E., Lesniewicz, K., Hassa, P. O., Hottiger, M. O., Litchfield, D. W., Shilton, B. H., Luscher, B. Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation. Molec. Cell 32: 57-69, 2008. 2. Lesniewicz, K., Luscher-Firzlaff, J., Poreba, E., Fuchs, P., Walsemann, G., Wiche, G., Luscher, B. Overlap of the gene encoding the novel poly(ADP-ribose) polymerase Parp10 with the plectin 1 gene and common use of exon sequences. Genomics 86: 38-46, 2005. 3. Yu, M., Schreek, S., Cerni, C., Schamberger, C., Lesniewicz, K., Poreba, E., Vervoorts, J., Walsemann, G., Grotzinger, J., Kremmer, E., Mehraein, Y., Mertsching, J., Kraft, R., Austen, M., Luscher-Firzlaff, J., Luscher, B. PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation. Oncogene 24: 1982-1993, 2005.

Uniprot ID: Q53GL7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 84875

Protein Name: Protein mono-ADP-ribosyltransferase PARP10

Gene Full Name: poly(ADP-ribose) polymerase family member 10

Synonyms: PARP10; Protein mono-ADP-ribosyltransferase PARP10; ADP-ribosyltransferase diphtheria toxin-like 10; ARTD10; Poly [ADP-ribose] polymerase 10; PARP-10