Anti-PAK2 Antibody Picoband®

SKU: A01419-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PAK2

Specificity:

Background: Serine/threonine-protein kinase PAK 2 is an enzyme that in humans is encoded by the PAK2 gene. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Immunogen: E.coli-derived human PAK2 recombinant protein (Position: M1-K288).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bokoch, G. M. Caspase-mediated activation of PAK2 during apoptosis: proteolytic kinase activation as a general mechanism of apoptotic signal transduction? Cell Death Diff. 5: 637-645, 1998. 2. Choi, J., Pease, D. R., Chen, S., Zhang, B., Phee, H. P21-activated kinase 2 is essential in maintenance of peripheral Foxp3+ regulatory T cells. Immunology 154: 309-321, 2018. 3. Eswaran, J., Soundararajan, M., Kumar, R., Knapp, S. UnPAKing the class differences among p21-activated kinases. Trends Biochem. Sci. 33: 394-403, 2008.

Uniprot ID: Q13177

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 58 kDa

Calculated Molecular Weight:

Gene ID: 5062

Protein Name: Serine/threonine-protein kinase PAK 2

Gene Full Name: p21 (RAC1) activated kinase 2

Synonyms: C t PAK2; Gamma PAK; p21 activated kinase 2; p27 PAK 2p34; p34; p58; PAK 2; PAK2; PAK65; PAKgamma; S6/H4 kinase; PAK-2