Anti-P53 Tp53 Monoclonal Antibody

SKU: MA1078

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,ICC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-2μg/ml, Human
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human

Gene Name: TP53

Specificity: No cross reactivity with other proteins.

Background: The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Immunogen: Recombinant human wild-type p53 protein.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Isobe, M.; Emanuel, B. S.; Givol, D.; Oren, M.; Croce, C. M. : Localization of gene for human p53 tumour antigen to band 17p13. Nature 320: 84-85, 1986. 2. Casey, G.; Yamanaka, Y.; Freiss, H.; Kobrin, M. S.; Lopez, M. E.; Buchler, M.; Beger, H. G.; Korc, M. : p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis. Cancer Lett. 69: 151-160, 1993.

Uniprot ID: P10361

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG2a

Phospho_site:

Clone Number: Clone: BP53-12

Observed Molecular Weight: 53 kDa

Calculated Molecular Weight: 43451 MW

Gene ID: Human(7157), Mouse(22059), Rat(24842)

Protein Name: Cellular tumor antigen p53

Gene Full Name: tumor protein p53

Synonyms: LFS1; TRP53; Li-Fraumeni syndrome; TRANSFORMATION-RELATED PROTEIN 53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53