Anti-P2Y12/P2ry12 Antibody Picoband®

SKU: A01136-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse

Gene Name: P2ry12

Specificity:

Background: P2Y12 is a chemoreceptor for adenosine diphosphate (ADP) that belongs to the Gi class of a group of G protein-coupled (GPCR) purinergic receptors. The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse P2Y12/P2ry12, which shares 73.3% and 93.3% amino acid (aa) sequence identity with human and rat P2Y12/P2ry12, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cattaneo, M., Zighetti, M. L., Lombardi, R., Martinez, C., Lecchi, A., Conley, P. B., Ware, J., Ruggeri, Z. M. Molecular bases of defective signal transduction in the platelet P2Y12 receptor of a patient with congenital bleeding. Proc. Nat. Acad. Sci. 100: 1978-1983, 2003. 2. Cattaneo, M. The platelet P2Y12 receptor for adenosine diphosphate: congenital and drug-induced defects. Blood 117: 2102-2112, 2011. 3. Cserep, C., Posfai, B., Lenart, N., Fekete, R., Laszlo, Z. I., Lele, Z., Orsolits, B., Molnar, G., Heindl, S., Schwarcz, A. D., Ujvari, K., Kornyei, Z., and 18 others. Microglia monitor and protect neuronal function through specialized somatic purinergic junctions. Science 367: 528-537, 2020.

Uniprot ID: Q9CPV9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 47643 MW

Gene ID: 70839

Protein Name: P2Y purinoceptor 12

Gene Full Name: purinergic receptor P2Y, G-protein coupled 12

Synonyms: ADP glucose receptor; ADPG R; HORK3; P2RY12; P2T(AC); P2Y purinoceptor 12; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; P2Y12 platelet ADP receptor; SP1999