Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)

SKU: MA1076

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ICC,WB

Application Details: Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 2-4μg/ml, Human, mouse, rat

Gene Name: CDKN1B

Specificity: No cross reactivity with other proteins.

Background: P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.

Immunogen: recombinant rodent p27Kip1 protein.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Chang, B.; Zheng, S. L.; Isaacs, S. D.; Wiley, K. E.; Turner, A.; Li, G.; Walsh, P. C.; Meyers, D. A.; Isaacs, W. B.; Xu, J. : A polymorphism in the CDKN1B gene is associated with increased risk of hereditary prostate cancer. Cancer Res. 64: 1997-1999, 2004. 2. Malek, N. P.; Sundberg, H.; McGrew, S.; Nakayama, K.; Kyriakidis, T. R.; Roberts, J. M. : A mouse knock-in model exposes sequential proteolytic pathways that regulate p27(Kip1) in G1 and S phase. Nature 413: 323-327, 2001. 3. Mitsuhashi, T.; Aoki, Y.; Eksioglu, Y. Z.; Takahashi, T.; Bhide, P. G.; Reeves, S. A.; Caviness, V. S., Jr. : Overexpression of p27(Kip1) lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. Proc. Nat. Acad. Sci. 98: 6435-6440, 2001.

Uniprot ID: O08769

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: DCS-72

Observed Molecular Weight: 18 kDa

Calculated Molecular Weight: 22139 MW

Gene ID: Human(1027), Mouse(12576), Rat(83571)

Protein Name: Cyclin-dependent kinase inhibitor 1B

Gene Full Name: cyclin-dependent kinase inhibitor 1B

Synonyms: KIP1; MEN4; CDKN4; MEN1B; P27KIP1; Cyclin-dependent kinase inhibitor p27