Anti-OSBP1 Antibody Picoband®

SKU: PA1949

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Rat,Mouse,Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: OSBP

Specificity: No cross reactivity with other proteins.

Background: OSBP (Oxysterol-Binding Protein), also called OSBP1, is a protein that in humans is encoded by the OSBP gene. Im et al. (2005) reported the structure of the full-length yeast oxysterol-binding-protein Osh4, a member of the OSBP-related protein (ORP) family, at 1.5- to 1.9-angstrom resolution in complexes with ergosterol, cholesterol, and 7-, 20- and 25-hydroxy cholesterol. Moreira et al. (2001) refined the localization of the OSBP1 gene to chromosome 11q12.1 by radiation hybrid analysis. Wang et al. (2005) found that OSBP functions as a cholesterol-binding scaffolding protein coordinating the activity of 2 phosphatases to control the extracellular signal-regulated kinase (ERK) signaling pathway. Cytosolic OSBP formed an approximately 440-kD oligomer with a member of the PTPPBS family of tyrosine phosphatases, the serine/threonine phosphatase PP2A, and cholesterol.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human OSBP1, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Im, Y. J., Raychaudhuri, S., Prinz, W. A., Hurley, J. H. Structural mechanism for sterol sensing and transport by OSBP-related proteins. Nature 437: 154-158, 2005. 2. Moreira, E. F., Jaworski, C., Li, A., Rodriguez, I. R. Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. J. Biol. Chem. 276: 18570-18578, 2001. 3. Wang, P., Weng, J., Anderson, R. G. W. OSBP is a cholesterol-regulated scaffolding protein in control of ERK1/2 activation. Science 307: 1472-1476, 2005.

Uniprot ID: P22059

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 89421 MW

Gene ID: 5007

Protein Name: Oxysterol-binding protein 1

Gene Full Name: oxysterol binding protein

Synonyms: OSBP; OSBP1; oxysterol binding protein; Oxysterol binding protein 1