Anti-ORC5L/ORC5 Antibody Picoband®

SKU: A03676-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: ORC5

Specificity:

Background: Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene. The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ORC5, identical to the related mouse sequence.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Frohling, S., Nakabayashi, K., Scherer, S. W., Dohner, H., Dohner, K. Mutation analysis of the origin recognition complex subunit 5 (ORC5L) gene in adult patients with myeloid leukemias exhibiting deletions of chromosome band 7q22. Hum. Genet. 108: 304-309, 2001. 2. Ishiai, M., Dean, F. B., Okumura, K., Abe, M., Moon, K.-Y., Amin, A. A., Kagotani, K., Taguchi, H., Murakami, Y., Hanaoka, F., O'Donnell, M., Hurwitz, J., Eki, T. Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22. Genomics 46: 294-298, 1997. 3. Quintana, D. G., Thome, K. C., Hou, Z., Ligon, A. H., Morton, C. C., Dutta, A. ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases. J. Biol. Chem. 273: 27137-27145, 1998.

Uniprot ID: O43913

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa

Calculated Molecular Weight: 28461 MW

Gene ID: 5001

Protein Name: Origin recognition complex subunit 5

Gene Full Name: origin recognition complex subunit 5

Synonyms: ORC5, ORC5L, ORC5P, ORC5T