Anti-NUMB Antibody Picoband®

SKU: A01206-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NUMB

Specificity:

Background: Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What’s more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.

Immunogen: E.coli-derived human NUMB recombinant protein (Position: E59-A567). Human NUMB shares 93.7% amino acid (aa) sequence identity with both mouse and rat NUMB.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dho SE, French MB, Woods SA, McGlade CJ (November 1999). Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain. J. Biol. Chem. 274 (46): 33097–104. 2. Katoh M, Katoh M (September 2006). NUMB is a break of WNT-Notch signaling cycle. Int. J. Mol. Med. 18(3): 517–21. 3. Pece S, Confalonieri S, R Romano P, Di Fiore PP (January 2011). NUMB-ing down cancer by more than just a NOTCH. Biochim. Biophys. Acta 1815 (1): 26–43.

Uniprot ID: P49757

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 72-75 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 8650

Protein Name: Protein numb homolog

Gene Full Name: NUMB endocytic adaptor protein

Synonyms: h Numb; NUMB; Protein numb homolog; Protein S171; S171