Anti-NSUN6 Antibody Picoband®

SKU: A13404-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NSUN6

Specificity:

Background: Enables tRNA (cytidine-5-)-methyltransferase activity and tRNA binding activity. Involved in tRNA C5-cytosine methylation. Located in cytoplasm. Implicated in autosomal recessive intellectual developmental disorder 82.

Immunogen: E.coli-derived human NSUN6 recombinant protein (Position: E40-E400). Human NSUN6 shares 82.3% amino acid (aa) sequence identity with mouse NSUN6.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chi, L., Delgado-Olguin, P. Expression of NOL1/NOP2/sun domain (Nsun) RNA methyltransferase family genes in early mouse embryogenesis. Gene Expr. Patterns 13: 319-327, 2013. 2. Gross, M. B. Personal Communication. Baltimore, Md. 11/10/2016. 3. Haag, S., Warda, A. S., Kretschmer, J., Gunnigmann, M. A., Hobartner, C., Bohnsack, M. T. NSUN6 is a human RNA methyltransferase that catalyzes formation of m(5)C72 in specific tRNAs. RNA 21: 1532-1543, 2015.

Uniprot ID: Q8TEA1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 52 kDa

Gene ID: 221078

Protein Name: tRNA

Gene Full Name: NOP2/Sun RNA methyltransferase 6

Synonyms: 4933414E04Rik; NOPD1; NSUN6