Anti-Nogo A/RTN4 Antibody Picoband®

SKU: A01844-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, human
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: RTN4

Specificity:

Background: Human neurite outgrowth inhibitor (NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nogo A/RTN4.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Prinjha R, Moore SE, Vinson M, Blake S, Morrow R, Christie G, Michalovich D, Simmons DL, Walsh FS.Inhibitor of neurite outgrowth in humans. Nature. 2000 Jan 27; 403(6768):383-4. 2. Chen MS, Huber AB, van der Haar ME, Frank M, Schnell L, Spillmann AA, Christ F, Schwab ME.Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1. Nature. 2000 Jan 27; 403(6768):434-9. 3. GrandPre T, Nakamura F, Vartanian T, Strittmatter SM.Identification of the Nogo inhibitor of axon regeneration as a Reticulon protein. Nature. 2000 Jan 27; 403(6768):439-44. 4. Fournier AE, GrandPre T, Strittmatter SM. Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration. Nature. 2001 Jan 18; 409(6818):341-6.

Uniprot ID: Q9NQC3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 180 kDa

Calculated Molecular Weight:

Gene ID: 57142

Protein Name: Reticulon-4

Gene Full Name: reticulon 4

Synonyms: ASY; Foocen; KIAA0886; Nbla00271; Nbla10545; Neurite outgrowth inhibitor; NI220/250; NOGO; NOGO A; Nogo B; Nogo C; Nogo protein; NOGOA; NOGOC; NSP; NSP CL; reticulon 4; Reticulon 5; RTN X; RTN4; RTN4 A; RTN4 B1; RTN4 B2; RTN4 C; SP1507