Anti-NFkB/NFKB1 p105/p50 Antibody Picoband®

SKU: PB9149

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: NFKB1

Specificity: No cross reactivity with other proteins.

Background: Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NFKB1 is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFKB1 proteins by p105 and generation of p50 by a cotranslational processing.

Immunogen: E.coli-derived human NFkB/NFKB1 p105/p50 recombinant protein (Position: M1-Q360). Human NFkB p105/P50 shares 93% amino acid (aa) sequence identity with mouse NFkB p105/P50.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Adler, A. S., Sinha, S., Kawahara, T. L. A., Zhang, J. Y., Segal, E., Chang, H. Y. Motif module map reveals enforcement of aging by continual NF-kappa-beta activity. Genes Dev. 21: 3244-3257, 2007. 2. Baek, S. H., Ohgi, K. A., Rose, D. W., Koo, E. H., Glass, C. K., Rosenfeld, M. G. Exchange of N-CoR corepressor and Tip60 coactivator complexes links gene expression by NF-kappa-B and beta-amyloid precursor protein. Cell 110: 55-67, 2002. 3. Huxford, T., Huang, D.-B., Malex, S., Ghosh, G. The crystal structure of the I-kappa-B-alpha complex reveals mechanisms of NF-kappa-B inactivation. Cell 95: 759-770, 1998.

Uniprot ID: P19838

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50 kDa, 120 kDa

Calculated Molecular Weight: 105356 MW

Gene ID: 4790

Protein Name: Nuclear factor NF-kappa-B p105 subunit

Gene Full Name: nuclear factor kappa B subunit 1

Synonyms: DNA binding factor KBF1; EBP 1; KBF1; NF kappa B; NFKB p105; NFKB p50; NFKB1; NFKB1; p105; NFKB1; p105; p50; nfkb1a; NF-κB; NF-κB 1; p105; p50