Anti-NF68 Nefl Antibody (Monoclonal, NR4)

SKU: MA1070

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Pig,Rat

Applications: IHC,IHC-F,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -
Western blot, 1-2μg/ml, Human, mouse, pig, rat

Gene Name: NEFL

Specificity: No cross reactivity with other proteins.

Background: Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Immunogen: Pig spinal cord.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. Zhu, Q.; Couillard-Despres, S.; Julien, J.-P. : Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp. Neurol. 148: 299-316, 1997. 2. Jordanova, A.; De Jonghe, P.; Boerkoel, C. F.; Takashima, H.; De Vriendt, E.; Ceuterick, C.; Martin, J.-J.; Butler, I. J.; Mancias, P.; Papasozomenos, S. C.; Terespolsky, D.; Potocki, L.; Brown, C. W.; Shy, M.; Rita, D. A.; Tournev, I.; Kremensky, I.; Lupski, J. R.; Timmerman, V. : Mutations in the neurofilament light chain gene (NEFL) cause early onset severe Charcot-Marie-Tooth disease. Brain 126: 590-597, 2003. 3. Previtali, S. C.; Zerega, B.; Sherman, D. L.; Brophy, P. J.; Dina, G.; King, R. H. M.; Salih, M. M.; Feltri, L.; Quattrini, A.; Ravazzolo, R.; Wrabetz, L.; Monaco, A. P.; Bolino, A. : Myotubularin-related 2 protein phosphatase and neurofilament light chain protein, both mutated in CMT neuropathies, interact in peripheral nerve. Hum. Molec. Genet. 12: 1713-1723, 2003.

Uniprot ID: P19527

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: NR4

Observed Molecular Weight: 72 kDa

Calculated Molecular Weight: 61335 MW

Gene ID: Human(4747), Mouse(18039), Rat(83613)

Protein Name: Neurofilament light polypeptide

Gene Full Name: neurofilament light chain

Synonyms: NFL; NF-L; NF68; CMT1F; CMT2E; 68 kDa neurofilament protein; Neurofilament triplet L protein