Anti-NDUFA9 Antibody Picoband®

SKU: A07420-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NDUFA9

Specificity:

Background: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 is an enzyme that in humans is encoded by the NDUFA9 gene. The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12.

Immunogen: E.coli-derived human NDUFA9 recombinant protein (Position: Q6-I377). Human NDUFA9 shares 78.9% and 76.4% amino acid (aa) sequence identity with mouse and rat NDUFA9, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Baens, M., Chaffanet, M., Cassiman, J.-J., van den Berghe, H., Marynen, P. Construction and evaluation of a hncDNA library of human 12 transcribed sequences derived from a somatic cell hybrid. Genomics 16: 214-218, 1993. 2. Baertling, F., Sanchez-Caballero, L., van den Brand, M. A. M., Fung, C.-W., Chan, S. H.-S., Wong, V. C.-N., Hellebrekers, D. M. E., de Coo, I. F. M., Smeitink, J. A. M., Rodenburg, R. J. T., Nijtmans, L. G. J. NDUFA9 point mutations cause a variable mitochondrial complex I assembly defect. Clin. Genet. 93: 111-118, 2018. 3. Fearnley, I. M., Finel, M., Skehel, J. M., Walker, J. E. NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Biochem. J. 278: 821-829, 1991.

Uniprot ID: Q16795

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight:

Gene ID: 4704

Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Gene Full Name: NADH:ubiquinone oxidoreductase subunit A9

Synonyms: CI 39kD; Complex I 39kD; NDUFA9; NDUFS2L; SDR22E1