Anti-NDUFA4 Antibody Picoband®

SKU: A09276-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NDUFA4

Specificity:

Background: NDUFA4, mitochondrial complex associated is a protein that in humans is encoded by the NDUFA4 gene. The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Immunogen: E.coli-derived human NDUFA4 recombinant protein (Position: M1-F81).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Zhang, Y. , Ge, M. , Chen, Y. , Yang, Y. , Chen, W. , & Wu, D. , et al. (2022). Ndufa4 promotes cell proliferation by enhancing oxidative phosphorylation in pancreatic adenocarcinoma. Journal of Bioenergetics and Biomembranes, 54(5), 283-291. 2. Xu, W. , Lai, Y. , Pan, Y. , Tan, M. , Ma, Y. , & Sheng, H. , et al. M6a rna methylation-mediated ndufa4 promotes cell proliferation and metabolism in gastric cancer. Cell Death & Disease. 3. Ramzan, R. , Rhiel, A. , Weber, P. , Kadenbach, B. , & Vogt, S. . (2019). Reversible dimerization of cytochrome c oxidase regulates mitochondrial respiration. Mitochondrion, 49.

Uniprot ID: O00483

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 12 kDa

Calculated Molecular Weight: 17081 MW

Gene ID: 4697

Protein Name: Cytochrome c oxidase subunit NDUFA4

Gene Full Name: cytochrome c oxidase associated subunit FA4

Synonyms: NDUFA4; Cytochrome c oxidase subunit NDUFA4; Complex I-MLRQ; CI-MLRQ; NADH-ubiquinone oxidoreductase MLRQ subunit