Anti-NCAPG Antibody Picoband®

SKU: A05501-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NCAPG

Specificity:

Background: This gene encodes a subunit of the condensin complex, which is responsible for the condensation and stabilization of chromosomes during mitosis and meiosis. Phosphorylation of the encoded protein activates the condensin complex. There are pseudogenes for this gene on chromosomes 8 and 15. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human NCAPG recombinant protein (Position: M1-R993).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Jager, D., Stockert, E., Jager, E., Gure, A. O., Scanlan, M. J., Knuth, A., Old, L. J., Chen, Y.-T. Serological cloning of a melanocyte rab guanosine 5-prime-triphosphate-binding protein and a chromosome condensation protein from a melanoma complementary DNA library. Cancer Res. 60: 3584-3591, 2000. 2. Xing, H., Wilkerson, D. C., Mayhew, C. N., Lubert, E. J., Skaggs, H. S., Goodson, M. L., Hong, Y., Park-Sarge, O.-K., Sarge, K. D. Mechanism of hsp70i gene bookmarking. Science 307: 421-423, 2005.

Uniprot ID: Q9BPX3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 131 kDa

Calculated Molecular Weight: 114 kDa

Gene ID: 64151

Protein Name: Condensin complex subunit 3

Gene Full Name: non-SMC condensin I complex subunit G

Synonyms: NCAPG; CAPG; NYMEL3; Condensin complex subunit 3; Chromosome-associated protein G; Condensin subunit CAP-G; hCAP-G; Melanoma antigen NY-MEL-3; Non-SMC condensin I complex subunit G; XCAP-G homolog