Anti-NAA60 Antibody Picoband®

SKU: A10429-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,IP,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: NAA60

Specificity:

Background: This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.

Immunogen: E.coli-derived human NAA60 recombinant protein (Position: M1-M242). Human NAA60 shares 97.1% and 97.5% amino acid (aa) sequence identity with mouse and rat NAA60, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chelban, V., Aksnes, H., Maroofian, R., LaMonica, L. C., Seabra, L., Siggervag, A., Devic, P., Shamseldin, H. E., Vandrovcova, J., Murphy, D., Richard, A. C., Quenez, O., and 48 others. Biallelic NAA60 variants with impaired N-terminal acetylation capacity cause autosomal recessive primary familial brain calcifications. Nature Commun. 15: 2269, 2024. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 9/22/2011. 3. Nakabayashi, K., Trujillo, A. M., Tayama, C., Camprubi, C., Yoshida, W., Lapunzina, P., Sanchez, A., Soejima, H., Aburatani, H., Nagae, G., Ogata, T., Hata, K., Monk, D. Methylation screening of reciprocal genome-wide UDPs identifies novel human-specific imprinted genes. Hum. Molec. Genet. 20: 3188-3197, 2011.

Uniprot ID: Q9H7X0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 27 kDa

Calculated Molecular Weight: 27 kDa

Gene ID: 79903

Protein Name: N-alpha-acetyltransferase 60

Gene Full Name: N-alpha-acetyltransferase 60, NatF catalytic subunit

Synonyms: NAA60; HAT4; NAT15; UNQ2771/PRO7155; N-alpha-acetyltransferase 60; hNaa60; EC 2.3.1.259; Histone acetyltransferase type B protein 4; HAT4; EC 2.3.1.48; N-acetyltransferase 15; N-alpha-acetyltransferase F; NatF