Anti-MYH6 Antibody Picoband®

SKU: A02808-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: MYH6

Specificity:

Background: Myosin heavy chain, α isoform (MHC-α) is a protein that in humans is encoded by the MYH6 gene. Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MYH6, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Buckingham, M., Alonso, S., Barton, P., Cohen, A., Daubas, P., Garner, I., Robert, B., Weydert, A. Actin and myosin multigene families: their expression during the formation and maturation of striated muscle. Am. J. Med. Genet. 25: 623-634, 1986. 2. Carniel, E., Taylor, M. R. G., Sinagra, G., Di Lenarda, A., Ku, L., Fain, P. R., Boucek, M. M., Cavanaugh, J., Miocic, S., Slavov, D., Graw, S. L., Feiger, J., Zhu, X. Z., Dao, D., Ferguson, D. A., Bristow, M. R., Mestroni, L. Alpha-myosin heavy chain: a sarcomeric gene associated with dilated and hypertrophic phenotypes of cardiomyopathy. Circulation 112: 54-59, 2005. 3. Ching, Y.-H., Ghosh, T. K., Cross, S. J., Packham, E. A., Honeyman, L., Loughna, S., Robinson, T. E., Dearlove, A. M., Ribas, G., Bonser, A. J., Thomas, N. R., Scotter, A. J., Caves, L. S. D., Tyrrell, G. P., Newbury-Ecob, R. A., Munnich, A., Bonnet, D., Brook, J. D. Mutation in myosin heavy chain 6 causes atrial septal defect. Nature Genet. 37: 423-428, 2005.

Uniprot ID: P13533

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 224 kDa

Calculated Molecular Weight: 96950 MW

Gene ID: 4624

Protein Name: Myosin-6

Gene Full Name: myosin heavy chain 6

Synonyms: alpha MHC; ASD3; MYH6; MYHC; MyHC alpha; MYHCA; Myosin 6; Myosin heavy chain 6