Anti-MYH14 Antibody Picoband®

SKU: A04528-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MYH14

Specificity:

Background: Myosin-14 is a protein that in humans is encoded by the MYH14 gene. This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human MYH14 recombinant protein (Position: M1-R1895). Human MYH14 shares 92.5% amino acid (aa) sequence identity with mouse MYH14.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Choi, B.-O., Kang, S. H., Hyun, Y. S., Kanwal, S., Park, S. W., Koo, H., Kim, S.-B., Choi, Y.-C., Yoo, J. H., Kim, J.-W., Park, K. D., Choi, K.-G., Kim, S. J., Zuchner, S., Chung, K. W. A complex phenotype of peripheral neuropathy, myopathy, hoarseness, and hearing loss is linked to an autosomal dominant mutation in MYH14. Hum. Mutat. 32: 669-677, 2011. 2. Donaudy, F., Snoeckx, R., Pfister, M., Zenner, H.-P., Blin, N., Di Stazio, M., Ferrara, A., Lanzara, C., Ficarella, R., Declau, F., Pusch, C. M., Nurnberg, P., Melchionda, S., Zelante, L., Ballana, E., Estivill, X., Van Camp, G., Gasparini, P., Savoia, A. Nonmuscle myosin heavy-chain gene MYH14 is expressed in cochlea and mutated in patients affected by autosomal dominant hearing impairment (DFNA4). Am. J. Hum. Genet. 74: 770-776, 2004. 3. Golomb, E., Ma, X., Jana, S. S., Preston, Y. A., Kawamoto, S., Shoham, N. G., Goldin, E., Conti, M. A., Sellers, J. R., Adelstein, R. S. Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. J. Biol. Chem. 279: 2800-2808, 2004.

Uniprot ID: Q7Z406

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight:

Gene ID: 79784

Protein Name: Myosin-14

Gene Full Name: myosin heavy chain 14

Synonyms: MHC16; MYH14; myosin; Myosin 14; Myosin heavy chain 14; myosin; heavy chain 14; NMHC II C; non-muscle II c