Anti-MPG Antibody Picoband®

SKU: PA1825

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: MPG

Specificity: No cross reactivity with other proteins.

Background: MPG (N-methylpurine-DNA glycosylase) also known as MDG, 3-METHYLADENINE DNA GLYCOSYLASE, 3MeAde DNA GLYCOSYLASE, AAG or APNG. The MPG gene is mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The MPG gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human MPG gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of Apng to the repair of several mutagenic lesions in vivo, Hang et al. (1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the Apng gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of APNG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the APNG-null animals.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MPG, different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Boosalis, M., Derfler, B., Call, K., Samson, L. Cloning of a human 3-methyladenine DNA glycosylase cDNA whose gene maps to human chromosome 16. (Abstract) Am. J. Hum. Genet. 49 (suppl.): 399 only, 1991. 2. Engelward, B. P., Weeda, G., Wyatt, M. D., Broekhof, J. L. M., de Wit, J., Donker, I., Allan, J. M., Gold, B., Hoeijmakers, J. H. J., Samson, L. D.Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase. Proc. Nat. Acad. Sci. 94: 13087-13092, 1997. 3. Kielman, M. F., Smits, R., Bernini, L. F. Localization and characterization of the mouse alpha-globin locus control region. Genomics 21: 431-433, 1994.

Uniprot ID: P29372

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight: 32869 MW

Gene ID: 4350

Protein Name: DNA-3-methyladenine glycosylase

Gene Full Name: N-methylpurine DNA glycosylase

Synonyms: 3 alkyladenine DNA glycosylase; AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1; MPG; N methylpurine DNA glycosylase; PIG11; PIG16