Anti-Mitofusin 1 MFN1 Antibody Picoband® (monoclonal, 3H3)

SKU: M02172-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Gene Name: MFN1

Specificity: No cross reactivity with other proteins.

Background: Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80-90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN-2, and to undergo ubiquitination by MARCH5. MFN-1 has two key domains. One is a coiled-coil region that mediates MFN-1: MFN-1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Adams MD, Kerlavage AR, Fields C, Venter JC (Sep 1993). 3,400 new expressed sequence tags identify diversity of transcripts in human brain. Nat Genet 4 (3): 256–67. 2. Meeusen, S., McCaffery, J. M., Nunnari, J. Mitochondrial fusion intermediates revealed in vitro. Science 305: 1747-1752, 2004. 3. Santel, A., Fuller, M. T. Control of mitochondrial morphology by a human mitofusin. J. Cell Sci. 114: 867-874, 2001.

Uniprot ID: Q8IWA4

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 3H3

Observed Molecular Weight: 84 kDa

Calculated Molecular Weight:

Gene ID: 55669

Protein Name: Mitofusin-1

Gene Full Name: mitofusin 1

Synonyms: Fzo homolog; hfzo1; hfzo2; MFN1; mitofusin 1; Transmembrane GTPase MFN1