Anti-MID2 Antibody Picoband®

SKU: A07086-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: MID2

Specificity:

Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MID2. Human MID2 shares 100% amino acid (aa) sequence identity with mouse MID2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Becker, M. A., Heidler, S. A., Bell, G. I., Seino, S., Le Beau, M. M., Westbrook, C. A., Neuman, W., Shapiro, L. J., Mohandas, T. K., Roessler, B. J., Palella, T. D. Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes. Genomics 8: 555-561, 1990. 2. Buchner, G., Montini, E., Andolfi, G., Quaderi, N., Cainarca, S., Messali, S., Bassi, M. T., Ballabio, A., Meroni, G., Franco, B. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum. Molec. Genet. 8: 1397-1407, 1999. 3. Geetha, T. S., Michealraj, K. A., Kabra, M., Kaur, G., Juyal, R. C., Thelma, B. K. Targeted deep resequencing identifies MID2 mutation for X-linked intellectual disability with varied disease severity in a large kindred from India. Hum. Mutat. 35: 41-44, 2014.

Uniprot ID: Q9UJV3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 83 kDa

Calculated Molecular Weight: 83 kDa

Gene ID: 11043

Protein Name: Probable E3 ubiquitin-protein ligase MID2

Gene Full Name: midline 2

Synonyms: FLJ37715; FLJ41813; FXY2; MID2; Midin 2; midline 2; Midline defect 2; RING finger protein 60; RNF60; TRIM1