Anti-METTL14 Antibody Picoband®

SKU: A07389-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IP,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: METTL14

Specificity:

Background: Methyltransferase like 14 is a protein that in humans is encoded by the METTL14 gene. METTL14 is part of a methyltransferase complex required for the common N6-methyladenosine (m6A) base modification that is enriched near stop codons and in long exons of mRNAs.

Immunogen: E.coli-derived human METTL14 recombinant protein (Position: Q12-D350).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/4/2015. 2. Liu, J., Yue, Y., Han, D., Wang, X., Fu, Y., Zhang, L., Jia, G., Yu, M., Lu, Z., Deng, X., Dai, Q., Chen, W., He, C. A METTL3-METTL14 complex mediates mammalian nuclear RNA N(6)-adenosine methylation. Nature Chem. Biol. 10: 93-95, 2014. 3. Nagase, T., Kikuno, R., Nakayama, M., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 273-281, 2000.

Uniprot ID: Q9HCE5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 108320 MW

Gene ID: 57721

Protein Name: N

Gene Full Name: methyltransferase 14, N6-adenosine-methyltransferase non-catalytic subunit

Synonyms: KIAA1627; methyltransferase like 14; METTL14