Anti-MDM2 Antibody Picoband®

SKU: PA1378-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: MDM2

Specificity: No cross reactivity with other proteins.

Background: Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al. (1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Uhrinova S, Uhrin D, Powers H, et al (2005). Structure of free MDM2 N-terminal domain reveals conformational adjustments that accompany p53-binding. J. Mol. Biol. 350 (3): 587–98.

Uniprot ID: Q00987

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90 kDa

Calculated Molecular Weight: 55233 MW

Gene ID: 4193

Protein Name: E3 ubiquitin-protein ligase Mdm2

Gene Full Name: MDM2 proto-oncogene

Synonyms: Double minute 2 protein; hdm2; HDMX; MDM2; Oncoprotein Mdm2; p53 binding protein Mdm2