Anti-MCAT Antibody Picoband®

SKU: A04806-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC/IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: MCAT

Specificity:

Background: The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human MCAT recombinant protein (Position: E37-R390).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gerber, S., Orssaud, C., Kaplan, J., Johansson, C., Rozet, J. M. MCAT mutations cause nuclear LHON-like optic neuropathy. Genes 12: 521, 2021. 2. Li, H., Yuan, S., Minegishi, Y., Suga, A., Yoshitake, K., Sheng, X., Ye, J., Smith, S., Bunkoczi, G., Yamamoto, M., Iwata, T. Novel mutations in malonyl-CoA-acyl carrier protein transacylase provoke autosomal recessive optic neuropathy. Hum. Molec. Genet. 29: 444-458, 2020. 3. Webb, B. D., Nowinski, S. M., Solmonson, A., Ganesh, J., Rodenburg, R. J., Leandro, J., Evans, A., Vu, H. S., Naidich, T. P., Gelb, B. D., DeBerardinis, R. J., Rutter, J., Houten, S. M. Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency. eLife 12: e68047, 2023.

Uniprot ID: Q8IVS2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight: 43 kDa

Gene ID: 7349

Protein Name: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial

Gene Full Name: malonyl-CoA-acyl carrier protein transacylase

Synonyms: MCT; Malonyl-CoA-acyl carrier protein transacylase; mitochondrial; FASN2C; fabD; EC:2.3.1.39