Anti-MARS1 Antibody Picoband®

SKU: A00216-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml/ml, -

Gene Name: MARS1

Specificity:

Background: This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.

Immunogen: E.coli-derived human MARS1 recombinant protein (Position: R25-E887).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Botta, E., Theil, A. F., Raams, A., Caligiuri, G., Giachetti, S., Bione, S., Accadia, M., Lombardi, A., Smith, D. E. C., Mendes, M. I., Swagemakers, S. M. A., van der Spek, P. J., Salomons, G. S., Hoeijmakers, J. H. J., Yesodharan, D., Nampoothiri, S., Ogi, T., Lehmann, A. R., Orioli, D., Vermeulen, W. Protein instability associated with AARS1 and MARS1 mutations causes trichothiodystrophy. Hum. Molec. Genet. 30: 1711-1720, 2021. 2. Cirullo, R. E., Wasmuth, J. J. Assignment of the human gene encoding methionyl-tRNA synthetase to chromosome 12. (Abstract) Cytogenet. Cell Genet. 37: 437 only, 1984. 3. Cirullo, R. E., Wasmuth, J. J. Assignment of the human MARS gene, encoding methioninyl-tRNA synthetase, to chromosome 12 using human x Chinese hamster cell hybrids. Somat. Cell Molec. Genet. 10: 225-234, 1984.

Uniprot ID: P56192

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 101 kDa

Calculated Molecular Weight: 101 kDa

Gene ID: 4141

Protein Name: Methionine--tRNA ligase, cytoplasmic

Gene Full Name: methionyl-tRNA synthetase 1

Synonyms: MARS, MARS1, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS